GnRH主动免疫雌性大鼠调控机制研究

doi:10.11843/j.issn.0366-6964.2016.03.011

Abstract

Abstract:

To explore the regulatory mechanisms of active immunization against GnRH in female SD rats.24 adult female SD rats were allocated to 3 groups of 8 animals each randomly.8 rats were immunized against GnRH at 12 week of age，4 weeks later an equal dose booster was administered.8 rats were surgically ovarictomy at 11 week of age while 8 intact rats without any treatments.Blood samples were collected at day 0(0 wpv) until just before sacrificed at 56 wpv.Serum levels of FSH，LH，E₂and P₄ were determined using RIA.After sacrificed，hypothalamus and pituitary were removed，the mRNA expressions of reproduction-related genes were determined by real-time fluorescence quantitative PCR(RT-PCR)；Ovaries were excised，adipose tissues were removed，then the mRNA expressions for reproduction-related genes were determined by RT-PCR.After immunization twice against GnRH，the concentrations of E₂，P₄，FSH and LH in immunized rats showed a significantly lower than that in Control group (P＜0.05).Moreover，active immunization against GnRH significantly down-regulated the mRNA levels of GnRH，GPR54，KiSS1and ER-α in the hypothalamus，GnRH-R，FSH-β and LH-β in the pituitary(P＜0.05)，as well as FSHR，LHR and ER-α in ovaries(P＜0.05).This study indicated GnRH had excellent immunogenic and was a good alternative of surgical castration.Active immunization against GnRH cause animals in a long-term infertility or sterility by the feedback loop of ER-α- GPR54/KiSS-1，and the fertility of immunocastrated rats show a tendancy to recover.

CLC Number:

S852.4

CAO Xiao-han，HAN Xing-fa，DU Xiao-gang，CHEN Zhi-yu，MENG Feng-yan，CHU Ming-xing，ZENG Xian-yin. Regulatory Mechanisms of Active Immunization against GnRH in Female Rats and Its Mechanism[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 502-508.

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Regulatory Mechanisms of Active Immunization against GnRH in Female Rats and Its Mechanism

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